Determination of Phospholipids in Milk by HPLC with Evaporative Light Scattering Detector: Optimization and Validation - Juniper Publishers
Determination of Phospholipids in Milk by HPLC with Evaporative Light Scattering Detector: Optimization and Validation Authored by Rodríguez-Otero JL Phospholipids are part of the milk fat globule membrane. Functional and technological properties make them interesting for the development of functional foods or as ingredients of technological interest. In this study an analytical procedure for determination of major phospholipids in milk (phosphatidyl Ethanolamine, phosphatidylinositol, phosphatidyl Choline, phosphatidyl Serine and sphingomyelin) by HPLC with evaporative light scattering detector was optimized and validated. The best calibrations were achieved when an exponential model was applied; the squared correlation coefficients show a satisfactory linearity ranging from 0.975 to 0.993. The limit of detection for the different phospholipids ranged from 0.17μg for phosphatidyl Serine to 0.61μg for phosphatidyl Ethanolamine; and the limit of quantification