Spatial and Temporal Quantification of the Morphofunctional Components of the Zebrafish kidneys-Juniper Publishers
JUNIPER PUBLISHERS - OPEN ACCESS JOURNAL OF DAIRY & VETERINARY SCIENCES Abstract Zebrafish is a small fish of the teleost family that has been widely used in animal experimentation to replace the use of rodents. It is characterized as a well defined study model capable of providing experimental information in several research areas. The present work aimed to analyze the spatiotemporal alterations of Zebrafish’s kidney architecture through the measurement of glomerular volume and stromal cell volume over thirteen weeks. We used 72 fish of the species Danio rerio, 6 months old, divided into thirteen groups, so that each group constituted one week. At the end of each week the euthanasia of the animals was promoted and the animals were fixed in aqueous solution of 10% formaldehyde, followed by conventional histological technique, cuts of 4 μm thickness and subsequent staining with Hematoxylin-Eosin. Photomicrographs of 3 random fields of each fragment were obtained...