Juniper Publishers Journal of Dairy and Veterinary Sciences

  Journal of Dairy & Veterinary Sciences - Juniper Publishers Abstract Evaluation of the concentrations of the sperm macromolecule called proAKAP4, has been successfully introduced as a pertinent sperm parameter to assess sperm quality and high concentrations of proAKAP4 was shown to be highly correlated with sperm motility and fertility in large mammals. The sandwich ELISA kits known as Pig 4MID® Kits allowed the artificial insemination stations to monitor sperm quality more accurately with threshold values qualifying each ejaculate and animal. Introducing new methods and procedures are always challenging and sperm frozen collections have been suggested to standardize sperm assessment in daily routine. We thus have designed an experimental study to assess proAKAP4 stability and integrity in neat frozen ejaculates. Following baseline measurements, fresh ejaculates were aliquoted and stored at -20 °C for stability experiments up to 6 months and following up to 10 freeze-thawing ...

  Journal of Dairy & Veterinary Sciences - Juniper Publishers Abstract Stem cell therapy is a growing field in veterinary medicine and has created a lot of hope for developing treatments for diseases that otherwise cannot be treated by traditional medical approaches. Clinical studies in animals show promising results indicating that stem cells may facilitate tissue repair and improve quality of life in pets, cats and horses. In this mini review, summarizes the current status of stem cell therapies in veterinary medicine describing the procedures used for isolation, characterization, administration of cells and treatment of various diseases that affect dogs, cats and horses. Keywords: Mesenchymal Stem Cells, Veterinary Medicine, Cell Therapy Introduction The field of stem cell research has attracted many investigatories in the past several years. Although stem cells have been known for some time, the biology of stem cells and their manipulation for therapeutic purposes have beco...

  Journal of Dairy & Veterinary Sciences - Juniper Publishers Abstract The temperature in a liquid nitrogen storage tank is critical to maintain the viability of bovine semen stored within the tank. The objective was to determine the relationship of the temperature gradient in the neck of a tank to the level of liquid nitrogen in the tank. Using an electronic thermometer, temperature was measured at three locations: a) 2 cm below the top of the neck, b) 7 cm below the top of the neck (and below the frost line), and c) 2 cm above the level of liquid nitrogen. Liquid nitrogen volume in the tank was incrementally decreased from 18 L to 6 L. The experiment was repeated twice, and temperatures at each location and nitrogen level were recorded four times. Data on the effect of liquid nitrogen level, location, and two-way interaction on the temperature gradient in the neck of the tank were analyzed using a general linear mixed model procedure. There was an effect of location and volum...