Fingerprinting of Lab Isolated From Silage, Water, Raw Milk, Starter Culture and Minas Artisanal Cheese from the Campo Das Vertentes Region, Brazil-Juniper Publishers
JUNIPER PUBLISHERS - OPEN ACCESS JOURNAL OF DAIRY & VETERINARY SCIENCES Abstract The aim of this study was to stablish phylogenetic relations of LAB isolated from water, silage, milk, endogenous starter culture and Minas artisanal cheese from Campo das Vertentes region. Eighty-eight Gram positive, catalase negative samples were submitted to 16-23S rDNA reaction, identifying 36 samples as belonging to Lactobacillus/Pediococcus genus. The 16S rRNA sequencing identified five species: Lactobacillus plantarum, L. paracasei, L. brevis, L. rhamnosus and Pediococcus acidilactici. The rep-PCR (GTG)5 fingerprinting showed predominance of L. plantarum (75.7%) in all sources, followed by L. brevis (9.1%), L. paracasei (6.1%), P acidilactici (6.1%) and L. rhamnosus (3%). Keywords: Milk analysis; Cattle; Reproduction; Urea nitrogen Abbreviations: CAPES: Coordenafao de Aperfeifoamento de Pessoal de Ni'vel Superior; FAPEMIG: Fundafao de Amparo a Pesquisa de Minas Gerai...