Effect of Melatonin and Vitamin E on SOD-Cu / Zn Levels and Membrane Permeability During Weed Semen Conservation - Juniper Publishers
Journal of Dairy & Veterinary Sciences Abstract In species such as the hair, oxidative stress is related to sperm damage during sperm conservation, which affects its fecundity. This research aimed to determine the effect of the addition of melatonin and vitamin E on the levels of Dismutasa-Cu / Zn Superoxide (SOD-Cu / Zn) during the conservation of diluted bristle semen. Seminal maestras of 7 to 18 months old verracos were collected. The optimal parameters are diluted and divided into aliquots: control (with diluent), control with vehicle (diluent and ethanol) and semen with melatonin (1.25 mM) and vitamin E (1 mM. at 16 °C, if enzyme activity (SOD-Cu / Zn), with commercial kit (Cayman®-Chemical Company Ann Arbor, MI, USA) and Hypo-osmotic Test (HOST +), to evaluate the integrity of Plasma membrane Results were analyzed by ANOVA, with SPSS 15.0 statistical package for Windows with p≤0.05 If SOD-Cu / Zn was observed in melatonin-based women, but significantly (p <0.05) higher r