Report on Isolation and Identification of Brucella Abortus from Aborted Foetus and Lymph Nodes of Beef Cattle in Malaysia - Juniper Publishers
Report on Isolation and Identification of Brucella Abortus from Aborted Foetus and Lymph Nodes of Beef Cattle in Malaysia
Authored by Zainor M Khairani-Bejo S
Isolation of living brucellae from tissues or organs such as aborted
foetus and lymph nodes has traditionally provided the most accurate
method for the detection of Brucella infection. The inoculated medium
was incubated at 37 °C for 7 days with 10% CO2. Bacterial colonies
growth on the agar was examined. The bacterial cells were stained with
Gram-staining and Modified Ziehl-Neelsen staining methods and later were
examined under microscope to determine their cell morphology.
Biochemical tests were performed to complete the phenotypic and
metabolic characterisation of the isolates. Brucella abortus were
isolated from stomach content, lung and cotyledon of the aborted foetus;
and supramammary and internal iliac lymph nodes of aborted cattle.
Histopathological examination of lymph nodes found the infiltrations of
microphages, neutrophils and giant cells with vacuolated and engorged
macrophages scattered in the necrotic debris of supramamarry lymph node
of aborted cattle.
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